2 Americans, 1 German win Nobel for super-zoom microscopes

(by Karl Ritter and Malin Rising, The Salt Lake Tribune) AP,  Stockholm — Two Americans and a German scientist won the Nobel Prize in chemistry Wednesday for finding ways to make microscopes more powerful than previously thought possible, allowing scientists to see how diseases develop inside the tiniest cells.

Working independently of each other, U.S. researchers Eric Betzig and William Moerner and Stefan Hell of Germany shattered previous limits on the resolution of optical microscopes by using glowing molecules to peer inside tiny components of life.

Their breakthroughs, starting in the 1990s, have enabled scientists to study diseases such as Parkinson’s, Alzheimer’s and Huntington’s at a molecular level, the Royal Swedish Academy of Sciences said.

“Due to their achievements the optical microscope can now peer into the nanoworld,” the academy said, giving the $1.1 million award jointly to the three scientists for “the development of super-resolved fluorescence microscopy.”

Betzig, 54, works at the Howard Hughes Medical Institute in Ashburn, Virginia. Hell, 51, is director of the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany, and also works at the German Cancer Research Center in Heidelberg. Moerner, 61, is a professor at Stanford University in California.

“I was totally surprised, I couldn’t believe it,” said Hell, who was born in Romania. “Fortunately, I [recognized] the voice of Nordmark and I realized it was real,” he added, referring to Staffan Nordmark, the academy’s permanent secretary.

The Nobel judges didn’t immediately reach Moerner, who was at a conference in Brazil. He found out about the prize from his wife after she was told by The Associated Press.

“I’m incredibly excited and happy to be included with Eric Betzig and Stefan Hell,” Moerner told the AP.

For a long time optical microscopes were limited by, among other things, the wavelength of light. So scientists believed they could never yield a resolution better than 0.2 micrometers.

But the three scientists were able to break that limit by using molecules that glow on command. The advance took optical microscopy into a new dimension that made it possible to study the interplay between molecules inside cells, including the aggregation of disease-related proteins, the academy said.

“We can at least dream now about being able to see within the cell on the molecular level, which is where all the action is,” Betzig said in a statement released by the Howard Hughes Medical Institute. “If we can do that, and study dynamics at that level, our understanding of cell biology and molecular biology should skyrocket.”

Hell has used these methods to study nerve cells to get a better understanding of brain synapses; Moerner has studied proteins related to Huntington’s disease; and Betzig has tracked cell division inside embryos, the academy said.

Hell said he was convinced that as a result of the discovery scientists will be able to find out more quickly what happens in a cell when a disease emerges.

“Any disease, in the end, can be boiled down to a malfunctioning of the cell,” he said. “And in order to understand what a disease actually means, you have to understand the cell and you have to understand the malfunction.”

Moerner said scientists can now tell whether individual molecules are different or the same.

“It’s very much like asking whether they all march to the same drummer or not,” Moerner told the AP. “When you can watch one by one, then we are able to observe exactly when it changes from one state to another.”

The technology lets scientists see “way below” the traditional limit for resolving detail with light microscopes, said Catherine Lewis, director of the cell biology and biophysics division of the National Institute of General Medical Sciences in Bethesda, Maryland.

“You can observe the behavior of individual molecules in living cells in real time. You can see … molecules moving around inside the cell. You can see them interacting with each other,” she said.

That gives a foundation for research into controlling those interactions in a way that may treat or diagnose disease, she said.

“You really need to be able to look at living cells because life is animate – it’s what defines life,” Betzig said.

Hell said that close look can shed light on disease. “Any disease, in the end, can be boiled down to a malfunctioning of the cell,” he said. “And in order to understand what a disease actually means, you have to understand the cell and you have to understand the malfunction.”]

Nobel committee member Claes Gustafsson called the laureates’ work “a revolution, because as recently as 15 years ago, it was believed to be theoretically impossible to break this barrier.”

Alex Chihak in Phoenix, Frank Jordans in Berlin and Malcolm Ritter in New York contributed to this report.

From the Associated Press.  Reprinted here for educational purposes only. May not be reproduced on other websites without permission from The Salt Lake  Tribune. Visit the website at sltrib .com.